The reusability and influence of three supports (calcium alginate, chitosan and polyurethane foam) in the biotransformation of cinnamyl alcohol by the spores and whole cells from the Colombian native fungus Colletotrichum acutatum was evaluated. The metabolic products cinnamaldehyde, 3-phenylpropanal, 3-phenyl-1-propanol, 1-phenylpropane-1,3-diol, 3-hydroxy-1-phenyl-1-propanone, hydrocinnamic acid, 2-phenylethanol, 1-phenylethane-1,2-diol, cinnamyl acetate, cinnamic acid and 3-phenyl propyl acetate were detected by GC-MS. The concentration of these products was clearly dependent on support. Thus, the bioprocesses with immobilized spores or whole cells into polyurethane foam were more efficient to obtain 1-phenylpropane-1,3-diol, and allowed reusing the support at least 3 or 4 times without significant loss of activity. However, under the same conditions, alginate and chitosan were inadequate supports for this purpose, and instead, 3-phenyl-1-propanol and cinnamic acid were the major metabolic compounds. Therefore, the biotransformation of cinnamyl alcohol using immobilized C. acutatum cells in polyurethane foam can potentially offer a simple and efficient way to obtain 1-phenylpropane-1,3-diol.